Potential use of toxic thermolabile proteins to study protein quality control systems.
نویسندگان
چکیده
SulA is an Escherichia coli division inhibitor with a short half-life whose accumulation results in filamentation. Here, we show that SulA is thermally unstable and forms aggregates at elevated temperatures. This property enables the selection of isolates with mutated protein quality control systems.
منابع مشابه
Toxic Thermolabile Proteins in the Study of Protein Quality Control
SulA is a division inhibitor of Escherichia coli with a short half life, whose accumulation results in filamentation. Here we show that SulA is thermally unstable and goes into aggregates at elevated temperatures. This property enables the selection of mutants in the protein quality control system.
متن کاملPlant-based expression systems for protein and antimicrobial peptide production
Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production in...
متن کاملSelecting appropriate hosts for recombinant proteins production: Review article
In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...
متن کاملThe intensity of electrophoretic bands containing egg non-vitellogenin derived proteins in relationship with embryo-larvae viability in common dentex (Dentex dentex) a preliminary evaluation
Despite the reported effects of egg vitellogenin-derived proteins (VtgDP) and amino acids on embryo-larvae viability in common dentex the effects of non-vitellogenin-derived proteins (non-Vtg-DP) has yet to be determined. As an initial study, fertilized eggs (70 batches) were provided by natural spawning of broodfish in captivity. Viability parameters (VP) such as egg floating, hatching, and la...
متن کاملA preliminary study on some potential toxic effects of Rosa damascena Mill
Rosa damascena has been traditionally used as an herbal medicine for different therapeutic purposes. Inorder to preliminarily study the probable toxic effects of the plant, its infusion was orally administered to 5groups of 5 dogs at doses 0.5-8 times that of human use in traditional medicine (90-1440 mg/kg/d) for 10successive days. The dogs in the control group (n=4) received placebo. Serum le...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 73 18 شماره
صفحات -
تاریخ انتشار 2007